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shuttle experiment's process

The Shuttle experiment

A proper description of biological processes at the atomic level require a full characterization of both the structure and the dynamics of biomolecules...

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DNP Solid

A Millikelvin Thermometer

The line shapes due to the quadrupolar couplings of deuterium spins allow the quick yet accurate determination of the deuterium spin temperature or, equivalently, the deuterium polarization, at absolute temperatures below ca. 10 mK.

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DNP Solid

Dynamic Nuclear Polarization at 9.4 T with Closed-Circuit Liquid Helium

‘‘Cryogen-free” DNP system composed of (1) a helium storage tank, (2) a 9.4 T magnet-VTI system with its DNP probe, and (3) a dry pump. Arrows illustrate the helium circuits involved to cool down the magnet, the VTI and the probe.

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DNP Solid

Hyperpolarizing Proteins by Exchange (“HYPEX”)

Hyperpolarized HN-CON correlation spectrum of ubiquitin in 98% deuterated buffer (red signals), superimposed on the corresponding thermal equilibrium spectrum obtained in 10% deuterated buffer (blue peaks). The former primarily show residues undergoing rapid solvent exchange, but also include several cases where the enhanced polarization has migrated to adjacent core residues.

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DNP Solid

Nitrogen-14 NMR by Double Cross-Polarization

Experimental (1H, 14N) correlation spectrum of a powdered sample of the self-assembled guanine G-tetrad (right) spinning at 62.5 kHz about the magic angle (MAS), obtained with double cross-polarization (DPC). Short- and long-range correlations can be detected. The overall experimental time was less than 54 minutes.

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DNP Solid

Hyperpolarization of Deuterated Drugs and Metabolites

Evolution of the populations of the deuterium spin states as a function of time, determined from experimental line intensities.

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DNP Solid

Thermal Mixing via Cross-Talk between Spin Reservoirs In DNP

Energy reservoirs involved in experiments that monitor the transfer between the polarizations of protons and 13C nuclei after the interruption of the microwave irradiation and saturation of one of the two nuclear reservoirs.

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DNP Solid

Towards Para-Water

Typical build-up and decay curves at room temperature that provide a measure of the lifetime of water as a molecular entity, when water is diluted in an aprotic solvent such as acetonitrile-d3. Red squares and orange triangles represent intensities of the peaks of H2O and HDO, respectively; green circles describe magnetization transfer from HDO to H2O, while blue rhombi describe the reverse reaction.

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DNP Solid

Sample Ripening affects Dynamic Nuclear Polarization

Build-up curves of the proton polarization induced by microwave saturation of the EPR transitions of 50 mm TEMPOL at 4.2 K and 6.7 T. Blue: sample vitrified immediately after preparation. Yellow: sample vitrified after a ripening for 45 min following sample preparation.

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DNP Solid

Sisyphus

Persephone supervising Sisyphus in the Underworld. Attica black-figure amphora, ca. 530 BC, Staatliche Antikensammlungen.

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histidine exchange

Fast Proton Exchange in Histidine

Owing to its imidazole side chain, histidine participates in various processes such as enzyme catalysis...

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Yttrium DNP

Kinetics of complexation of metal ions by DNP

Kinetics of Yttrium-Ligand Complexation Monitored Using Hyperpolarized 89Y as a Model for Gadolinium in Contrast Agents...

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DNP Ribosome

Enhancement of NMR signals of ribosomes by DNP

The impact of NMR on studies of large macromolecular complexes hinges on...

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Methyl

Methyl group relaxation

Over the past two decades, methyl groups have been shown to be a valuable probes of protein sidechain dynamics through the measurement of spin relaxation...

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hiAPP

Amyloid proteins: structure and dynamics

The formation of protein amyloid deposits is associated with major human diseases including Alzheimer’s disease...

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MRI

MRI in inhomogeneous magnetic fields

Image of a glass spiral phantom surrounded by water inserted into a tube with 3.8 mm inner diameter ...

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DNP Dissolution

Enhancement of NMR signals by ‘dissolution’ DNP

The efficiency of dissolution dynamic nuclear polarization can be boosted by Hartmann-Hahn cross polarization...

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DNP Solid

Dynamic nuclear polarization (DNP) in solids

Performing solid-state DNP experiments at high magnetic fields requires several costly accessories in addition to a normal MAS setup...

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Solid

Narrow Lines in Solid-State Nuclear Magnetic Resonance

Efficient heteronuclear decoupling is vital for obtaining high-resolution solid-state NMR spectra of low-gamma nuclei such as carbon-13.

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Diffusion Coeff

Determination of diffusion coefficients of macromolecules in solution

Pulse sequence for Signal Optimization with Recovery in Diffusion Delays (SORDID), designed for the efficient measurement of slow diffusion coefficients.

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Internal mobility

Internal mobility in proteins

Identification of chemical exchange contributions to the relaxation of 1H/15N multiple-quantum coherences of NHN pairs in ubiquitin.

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fast motion

Characterization of very fast motions in solid peptides

Experimental double-quantum 14N/1H correlation spectrum of the -NH3 group (I) and the two rigid amide NH pairs (II) in the tripeptide Ala-Ala-Gly.

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Enzym DNP

Kinetics of enzymatic reactions by ‘dissolution’ DNP

Dissolution dynamic nuclear polarization (D-DNP) experiments enabled us to study the kinetics of the enzymatic phosphorylation reaction of glucose to form glucose-6-phosphate (G6P) by hexokinase (HK), with or without the presence of an excess of G6P, which is known to be an inhibitor of the enzyme.

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DNP Solid

Indirect detection of nitrogen-14 NMR in solids

Two-dimensional (2D) spectra of a polycrystalline histidine sample spinning at 62.5 kHz in a static field of 18.8 T (800 MHz for protons),

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DNP Solid

Recording single-scan two-dimensional spectra in a fraction of a second

Single-scan 2D proton spectrum of quinidine dissolved in CDCl3 recorded in less than 0.2 s using a single-scan experiment with adiabatic encoding pulses and alternating decoding gradients.

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