Fast Proton Exchange in Histidine

Determination of fast exchange rates up to 100 000 jumps per second of labile protons by indirect detection of the decay of nitrogen-15 magnetization

Protonexchange Histidine Shem

Abstract

Histidine participates in various processes such as enzyme catalysis, pH regulation, metal binding, and phosphorylation. The very fast exchange rates of labile protons in an aqueous solution were obtained by indirect detection of the decay of transverse nitrogen-15 magnetization. Lifetimes as short as 12 microseconds could be determined. The chemical shifts and scalar coupling constants of the invisible exchanging protons could also be determined accurately.

Related Publications

  • Measuring Fast Hydrogen Exchange Rates by NMR Spectroscopy. F. Kateb, P. Pelupessy, and G. Bodenhausen, J. Magn. Reson. 184, 108-113 (2007).
  • Exchange Rate Constants of Invisible Protons in Proteins Determined by NMR Spectroscopy. T. Segawa, F. Kateb, L. Duma, G. Bodenhausen, and P. Pelupessy, ChemBioChem, 9, 537-542 (2008).
  • Fast Proton Exchange in Histidine: Measurement of Rate Constants Through Indirect Detection by NMR Spectroscopy. A. A. Sehgal, L. Duma, G. Bodenhausen, and P. Pelupessy, Chem. Eur. J. 20, 6332-6338 (2014) + Supplement.
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